Journal: Parasite Immunology

Article Title: Cortisol Regulates PD‐1 and IL‐12 in Canine Leishmaniasis

doi: 10.1111/pim.70062

Figure Lengend Snippet: Expression of iNOS and Arginase‐1 enzymes in PBMCs from healthy dogs (Healthy) and dogs with leishmaniasis (Leishmaniasis). Expression levels of iNOS (A) and Arginase‐1 (B) were measured in PBMCs from healthy dogs (healthy group, n = 10) and dogs with leishmaniasis (leishmaniasis group, n = 13) by flow cytometry. Data are presented as the median and interquartile range (25th and 75th percentiles). Symbols represent individual data points for each animal. Mann–Whitney test was performed for group comparisons for iNOS and unpaired t ‐test was performed for group comparisons for Arginase‐1. Asterisks indicate significant differences (* p < 0.05, ** p < 0.01).

Article Snippet: The cells were then resuspended with 50 μL of permeabilization buffer (Invitrogen, CA, USA) and incubated with PE‐conjugated rabbit anti‐mouse iNOS polyclonal antibody (BIORBYT, UK) and FITC‐conjugated rabbit anti‐human arginase 1 polyclonal antibody (BIORBYT, UK) for 60 min at 26°C.

Techniques: Expressing, Flow Cytometry, MANN-WHITNEY